ABSTRACT
Treatment of rats for three successive days with bromazepam, loxapine and diazepam in doses of 5, 10, and 20 mg/kg body weight, respectively, caused a marked increase in microsomal drug-metabolizing enzyme activity involved in N-demethylation and aromatic p-hydroxylation. In addition, these drugs significantly decreased the sleeping time of animals induced by hexabarbital [100 mg/kg. I.P.]. Sulpiride, a neuroleptic agent, at a dose of 15 mg/kg, I.P. was without effect on both the microsomal drug-metabolizing enzymes and the hexobarbital sleeping time. With regards to the effect of these drugs on mitochondrial energetics, bromazepam [0.1 mM] and diazepam [0.1 mM] significantly inhibited ATPase activity, loxapine [0.1 mM] stimulated ATPase activity and decreased ADP : O ratio, whereas sulpiride was without effect. The significance of these results was discussed